Review



total cd3 cd4 t cell fractions  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    ATCC total cd3 cd4 t cell fractions
    ( A ) Purified <t>CD3</t> + <t>CD4</t> + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.
    Total Cd3 Cd4 T Cell Fractions, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+cd3+cd4+t+cell+fractions/Primary+CD4%2B+Helper+T+Cells/pmc02908536-149-0-25
    Average 95 stars, based on 806 article reviews
    total cd3 cd4 t cell fractions - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)"

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0011469

    ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.
    Figure Legend Snippet: ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Techniques Used: Purification, Labeling, Cell Culture, Derivative Assay, Flow Cytometry, Expressing, Co-Culture Assay

    ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.
    Figure Legend Snippet: ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Techniques Used: Flow Cytometry, Expressing, Purification, Cell Culture, Staining, Derivative Assay, Blocking Assay

    Related Articles

    Isolation:

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)
    Article Snippet: .. Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 μg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 μg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates. ..

    Cell Culture:

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)
    Article Snippet: .. Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 μg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 μg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates. ..



    Similar Products

    total cd3 cd4 t cell fractions  (ATCC)
    95
    ATCC total cd3 cd4 t cell fractions
    ( A ) Purified <t>CD3</t> + <t>CD4</t> + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.
    Total Cd3 Cd4 T Cell Fractions, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+cd3+cd4+t+cell+fractions/Primary+CD4%2B+Helper+T+Cells/pmc02908536-149-0-25
    Average 95 stars, based on 1 article reviews
    total cd3 cd4 t cell fractions - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    doi: 10.1371/journal.pone.0011469

    Figure Lengend Snippet: ( A ) Purified CD3 + CD4 + T cells were labeled with CFSE and cultured as described in ± TMV or DC-derived MV (5 µg/mL). On days 3, 5 and 8, the frequency of CD4 + CD25 + FOXP3 + Treg among proliferating T cells was determined by flow cytometry. The data (means ± SD) represent three independent experiments (*p<0.01). ( B ) Proliferating CD3 + CD4 + T cells (squares) were tested for co-expression of CD25 in a representative co-culture ± TMV. A higher proportion of proliferating CD4 + T cells expressed CD25 in the co-culture with TMV than without TMV. ( C ) The proliferating CD4 + CD25 + T cells in the co-cultures with TMV were evaluated for the frequency of FOXP3 + T cells upon gating on the CD4 + CD25 high subset (see box). Over 90% of these cells also expressed intracellular FOXP3. Data are representative for one out of 6 cultures tested.

    Article Snippet: Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 µg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 µg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates.

    Techniques: Purification, Labeling, Cell Culture, Derivative Assay, Flow Cytometry, Expressing, Co-Culture Assay

    ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Microvesicles Induce, Expand and Up-Regulate Biological Activities of Human Regulatory T Cells (Treg)

    doi: 10.1371/journal.pone.0011469

    Figure Lengend Snippet: ( A ) Flow cytometry analysis of TGF-β1 and IL-10 expression on TMV purified from OVCAR-3 SN and coated onto latex beads. ( B ) CD4 + CD25 high FOXP3 + T cells were cultured with OKT3, anti-CD28 and IL-2 (150 IU/mL) +/− TMV for 72 h at 37°C in the presence of Golgistop and then stained for CD4, CD3, CD25 and intracellular TGF-β1 and IL-10. Expression of both cytokines was up-regulated in the presence of TMV (p<0.05). ( C ) SMAD2/3 and STAT3 phosphorylation in Treg before and after exposure to TMV. Representative results are from one of three independent experiments for A , B and C . ( D ) The percentage of CD4 + CD25 high FOXP3 + T cells increased among CD4 + CD25 + T cells cultured in the presence of TMV but not DC-derived MV. Neutralizing anti-TGF-β1 and/or anti-IL-10 Abs inhibited the induction of Treg by TMV. Non-blocking IgG isotype control Abs were used as controls. Asterisks indicate decreases (p<0.05) in Treg percentages in the presence of neutralizing Abs. Results are means ± SD of three independent experiments.

    Article Snippet: Total CD3 + CD4 + T cell fractions or isolated CD4 + CD25 + cells were cultured in AIMV medium with plate-bound OKT3 (1 µg/mL; American Type Culture Collection), soluble anti-CD28 Abs (1 µg/mL) and IL-2 (150 IU/mL) at 37°C/5%CO 2 in wells of 96-wells plates.

    Techniques: Flow Cytometry, Expressing, Purification, Cell Culture, Staining, Derivative Assay, Blocking Assay